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AtTaq DNA Polymerase

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産品名稱: AtTaq DNA Polymerase
産品型號: PL3201 PL3202
産品展商: Vivantis Technologies Sdn. Bhd.
美元($)價格: 0.00 元
歐元()價格: 0.00 元
人民幣(¥)價格: 0.00 元
産品文檔: 無相關文檔
發布時間:2020-10-27

簡單介紹

Home DNA Amplification Products AtTaq DNA Polymerase (Hot Start) AtTaq DNA Polymerase (Hot Start) Description AtTaq DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA Polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. AtTaq DNA Polymerase catalyzes the polym


AtTaq DNA Polymerase  的詳細介紹
Home  DNA Amplification Products  AtTaq DNA Polymerase (Hot Start)

AtTaq DNA Polymerase (Hot Start)

Description
AtTaq DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA Polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. AtTaq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg?+ and has the 5' to 3' exonuclease activity.

Features

  • Ultra pure recombinant protein which is reversibly complex with anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures.
  • Carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C.
  • Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic “Hot Start” amplification, which allows for the assembly of amplification reactions at room temperature.
  • High stability of the complexes allows enormous increase in amplification specificity, sensitivity and yield in comparison to the conventional amplification assembly method.
  • Increased specificity as a result of reduced amplification artefacts such as primer-dimer formation and mispriming in multiplex amplification.
  • 10X ViBuffer S provided for amplification of more than 5kb amplicon.

Unit Definition 
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [?H]dTTP), 10μg activated calf thymus DNA and 100μg /ml BSA in a final volume of 50μl.

Supplied With

  • 10X ViBuffer A (without MgCl2 ) 
    500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton? X- 100. The buffer is optimized for use with 0.1-0.2mM of each dNTP.
  • 10X ViBuffer S 
    160mM (NH4)2SO4 , 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton? X-100. The buffer is optimized for use with 0.35mM of each dNTP.
  • 50mM MgCl2

Quality Control 
All preparations are *****ed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
20mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.5% Tween? 20, 0.5% Nonidet- P40, 0.1mM EDTA, 1mM DTT, and 50% glycerol. Store at -20°C.

Ordering Information

Catalog No Description Pack Size
PL3201 AtTaq DNA Polymerase 200u, 5u/μl
PL3202 AtTaq DNA Polymerase 500u, 5u/μl

Download
Manual

AtTaq DNA Polymerase

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