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Chromo Pfu DNA Polymerase

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産品名稱: Chromo Pfu DNA Polymerase
産品型號: PL5205 PL5206
産品展商: Vivantis Technologies Sdn. Bhd.
美元($)價格: 0.00 元
歐元()價格: 0.00 元
人民幣(¥)價格: 0.00 元
産品文檔: 無相關文檔
發布時間:2020-10-27

簡單介紹

Home DNA Amplification Products Chromo Pfu DNA Polymerase (Pyrococcus furiosus) Chromo Pfu DNA Polymerase (Pyrococcus furiosus) Description Chromo Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' dire


Chromo Pfu DNA Polymerase  的詳細介紹
Home  DNA Amplification Products  Chromo Pfu DNA Polymerase (Pyrococcus furiosus)

Chromo Pfu DNA Polymerase (Pyrococcus furiosus)

Description
Chromo Pfu DNA Polymerase is an extremely thermostable proofreading DNA polymerase, suitable for applications requiring high temperatures synthesis of DNA. Pfu DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+. It exhibits the 3' to 5' proofreading activity. The enzyme is supplemented with inert color tracer dyes.

Features

  • Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
  • Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
  • Ultra pure recombinant protein allows amplification up to 8kb
  • 5X ViBuffer Pfu provided for both short and long amplification.
  • Recommended for use in high-fidelity amplification and cloning of blunt-ended amplification products.

Unit Definition 
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2 , 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [?H]dTTP), 10μg activated calf thymus DNA and 100μg/ml BSA in a final volume of 50μl.

Supplied With

  • 50mM MgCl2
  • 5X ViBuffer Pfu

Quality Control 
All preparations are *****ed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.

Storage Buffer 
50mM Tris-HCl (pH 8.0 at 22°C ), 100mM KCl, 0.1% Tween? 20, 0.1% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.

Amplification of 1.5kb DNA fragment using Chromo Pfu DNA Polymerase with 10X ViBuffer A (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Chromo Pfu DNA Polymerase
2. PCR product using 1U of Chromo Pfu DNA Polymerase
3. PCR product using 0.5U of Chromo Pfu DNA Polymerase
4. PCR product using 0.25U of Chromo Pfu DNA Polymerase


Amplification of 1.5kb DNA fragment from Lambda DNA using Chromo Pfu DNA Polymerase with 10X ViBuffer S (Lane A1-A4) and 5X ViBuffer Pfu (Lane B1-B4).

1. PCR product using 2U of Chromo Pfu DNA Polymerase
2. PCR product using 1U of Chromo Pfu DNA Polymerase
3. PCR product using 0.5U of Chromo Pfu DNA Polymerase

Ordering Information

Catalog No Description Pack Size
PL5205 Chromo Pfu DNA Polymerase 100u, 1u/μl
PL5206 Chromo Pfu DNA Polymerase 500u, 1u/μl

Download
Manual

Chromo Pfu DNA Polymerase

Comparison Test Report

Chromo Pfu DNA Polymerase

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