主營産品: ad檢測試劑盒,agc試劑盒,akt試劑盒,ald檢測試劑盒,ctc試劑盒,hp試劑盒,apo試劑盒,chip試劑盒,mmp試劑盒,酶聯免疫,酶聯免疫ELISA,酶聯免疫法,elisa原理,酶聯免疫試劑盒,elisa酶免試劑盒,Antibody,抗體
産品詳情

Flex-C Cloning Kit

如果您對該産品感興趣的話,可以
産品名稱: Flex-C Cloning Kit
産品型號: MEPL01 MEPL02
産品展商: Vivantis Technologies Sdn. Bhd.
美元($)價格: 0.00 元
歐元()價格: 0.00 元
人民幣(¥)價格: 0.00 元
産品文檔: 無相關文檔
發布時間:2020-10-27

簡單介紹

Home Cloning Kit Flex-C Cloning Kit Flex-C Cloning Kit Description Flex-C Cloning Kit is a highly efficient, rapid, and easy-to-use PCR cloning kit. The Flex-C Enzyme allows direct cloning of any PCR fragments into any linearized expression vector at any site in a single 20-minute reaction. The application protocol is simple. The PCR fragments can be generated by PCR Polymerases (for eg. T


Flex-C Cloning Kit  的詳細介紹
Home  Cloning Kit  Flex-C Cloning Kit

Flex-C Cloning Kit

Description
Flex-C Cloning Kit is a highly efficient, rapid, and easy-to-use PCR cloning kit. The Flex-C Enzyme allows direct cloning of any PCR fragments into any linearized expression vector at any site in a single 20-minute reaction.
The application protocol is simple. The PCR fragments can be generated by PCR Polymerases (for eg. Taq DNA Polymerase) with primers that are designed to have at least 10 bases of homology with the vector at their linear ends. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The linearized vector can be generated by PCR or restriction enzymes (single or double digestion). Flex-C Enzyme joins PCR fragments and linearized vectors accurately and efficiently by recognizing the 10bp overlap at their ends.
This method allows cloning of multiple fragments into a single vector in a single reaction, without subcloning, to create fusion proteins, to delete and replace DNA sequences or to insert point mutations. The Flex-C Cloning Kit is highly efficient with a 95% insert rate. The Flex-C Cloning Kit also includes Vivantis Taq DNA Polymerase and reaction buffers as well as dNTPs for subsequent PCR screening of clones.

Features

  • Clone any insert, at any site within any vector
  • Restriction enzyme, phosphatase and ligase-free system
  • Joining multiple fragments at once
  • Broad PCR size up to 10kb
  • Good for 5’ overhangs, 3’ overhangs, blunt ends
  • Precise insertion at a desired orientation
  • High efficiency with > 95% positive clones
  • Multiple applications: 
    -Adding adaptor, linker and tag before or after the insert
    -Mutation generation
    -Gene synthesis
  • High throughput application

Kit Components
Flex-C Cloning Kit (Without Competent Cell)

  • Flex-C Cloning Enzyme
  • Taq DNA Polymerase and buffers
  • 10mM dNTP Mix
  • Nuclease-free water

Flex-C Cloning Kit (With Competent Cell)

  • Flex-C Cloning Enzyme
  • Competent Cells (1X109cfu/?g)
  • Taq DNA Polymerase and buffers
  • 10mM dNTP Mix
  • Nuclease-free water

Storage & Stability
Store at -20°C.

Ordering Information

Catalog No Description Pack Size
MEPL01 Flex-C Cloning Kit (Without Competent Cell) 20 applications
MEPL02 Flex-C Cloning Kit (With Competent Cell) 20 applications

Download
Manual

Flex-C Cloning Kit (Without Competent Cell)
Flex-C Cloning Kit (With Competent Cell)

 
産品留言
標題
聯系人
聯系電話
內容
附件:
附件支持RAR,JPG,PNG格式,大小在2M範圍,只允許上傳1個
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發送信息!
2.如有必要,請您留下您的詳細聯系方式!
Copyright@ 2003-2021  上海拜力生物科技有限公司  版權所有  

本公司網站所展示銷售的産品僅供科研!

            

沪公网安备 31011202007337号